Research Paper Volume 13, Issue 3 pp 4063—4078

PRPS1-mediated purine biosynthesis is critical for pluripotent stem cell survival and stemness

Purine metabolite levels are high in pluripotent stem cells. (A) Heatmap showing metabolomics analysis in PSCs, compared with somatic cell control using UHPLC-MS. Somatic cells, human fibroblast cells; iPS, induced pluripotency stem cells from cord blood. (B, C) Comparison of the absolute levels of NTPs (B) and dNTPs (C) in PSCs and somatic cells by analyzing analyte peak area (counts x 106/106 cells) from (A). (D) Heatmap showing the metabolites except for purine synthesis-related in PSCs and somatic cells. In (C) data are expressed as the mean ± SD. *P

Figure 1. Purine metabolite levels are high in pluripotent stem cells. (A) Heatmap showing metabolomics analysis in PSCs, compared with somatic cell control using UHPLC-MS. Somatic cells, human fibroblast cells; iPS, induced pluripotency stem cells from cord blood. (B, C) Comparison of the absolute levels of NTPs (B) and dNTPs (C) in PSCs and somatic cells by analyzing analyte peak area (counts x 106/106 cells) from (A). (D) Heatmap showing the metabolites except for purine synthesis-related in PSCs and somatic cells. In (C) data are expressed as the mean ± SD. *P <0.05, by One-way ANOVA. Data are representative of three independent experiments with similar results.