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Research Paper Volume 13, Issue 3 pp 4079—4095
Microglia exosomal miRNA-137 attenuates ischemic brain injury through targeting Notch1
Figure 1. M2-phenotype microglia-conditioned medium attenuated oxygen-glucose deprivation (OGD)-induced neuronal death. (A) mRNA expressions of CD206 and ARG in BV2 cells treated with or without IL-4 treatment. (B) Protein expressions of CD206 and ARG in BV2 cells treated with or without IL-4 treatment. (C) TUNEL assay for detecting apoptosis in neurons in 1) control, 2) OGD, 3) OGD plus M2-phenotype microglia-conditioned medium (OGD+BV2-CM), and 4) OGD plus M2-phenotype microglia-conditioned medium plus exosome secretion inhibitor GW4869 (OGD+BV2-CM+GW4869). Scale bar = 20 μm. (D) Lactate dehydrogenase (LDH) assay for detecting LDH activity in neurons in 1) control, 2) OGD, 3) OGD+BV2-CM, and 4) OGD+BV2-CM+GW4869. Data are presented as mean±SD. *, p<0.05. At least three replicates were available for statistical analysis in each treatment.