Research Paper Volume 13, Issue 3 pp 4079—4095

Microglia exosomal miRNA-137 attenuates ischemic brain injury through targeting Notch1

MiRNA-137 was upregulated in M2-phenotype microglia-derived exosomes (BV2-Exo). (A, B). Heatmap and volcano plot of expression profiles for differentially expressed miRNAs between BV2-Exo and exosomes derived from control BV2 cells (BV2 Exo-CN), as determined by RNA-seq analysis. (C) Expression of miRNA-137 in 1) BV2 Exo-CN, 2) BV2-Exo, 3) BV2-Exo derive from BV2 cells treated with miRNA-137 inhibitor (BV2-Exo+miRNA-137-IN), and 4) BV2-Exo derive from BV2 cells treated with miRNA-137 inhibitor negative control (BV2-Exo+miRNA-137-NC), as detected by RT-PCR. (D) Expression of miRNA-137 in neurons treated with 1) control, 2) OGD, 3) OGD plus BV2-Exo, 4) OGD plus BV2-Exo+miRNA-137-IN, and 5) OGD plus BV2-Exo+miRNA-137-NC, as detected by RT-PCR. Data are presented as mean±SD. *, p

Figure 4. MiRNA-137 was upregulated in M2-phenotype microglia-derived exosomes (BV2-Exo). (A, B). Heatmap and volcano plot of expression profiles for differentially expressed miRNAs between BV2-Exo and exosomes derived from control BV2 cells (BV2 Exo-CN), as determined by RNA-seq analysis. (C) Expression of miRNA-137 in 1) BV2 Exo-CN, 2) BV2-Exo, 3) BV2-Exo derive from BV2 cells treated with miRNA-137 inhibitor (BV2-Exo+miRNA-137-IN), and 4) BV2-Exo derive from BV2 cells treated with miRNA-137 inhibitor negative control (BV2-Exo+miRNA-137-NC), as detected by RT-PCR. (D) Expression of miRNA-137 in neurons treated with 1) control, 2) OGD, 3) OGD plus BV2-Exo, 4) OGD plus BV2-Exo+miRNA-137-IN, and 5) OGD plus BV2-Exo+miRNA-137-NC, as detected by RT-PCR. Data are presented as mean±SD. *, p<0.05. At least three replicates were available for statistical analysis in each treatment.