Research Paper Volume 13, Issue 1 pp 1422—1439

Functional characterization of DLK1/MEG3 locus on chromosome 14q32.2 reveals the differentiation of pituitary neuroendocrine tumors

The protein-coding RNA landscape in 172 PitNETs. (A) Heatmap of unsupervised hierarchical clustering of the top 352 most variable genes among 31 somatotroph adenomas, 17 lactotroph adenomas, 79 gonadotroph adenomas and 45 corticotroph adenomas (|log2FC|>2, adj.P.valB–D) Volcano plots showing the significantly differentially expressed genes among somatotroph adenomas vs. 3 other subtypes of PitNETs (up: red, down: blue), all log2FC of DLK1 > 7. (E) Venn diagram showing the intersection of DEGs among somatotroph adenomas vs. 3 other subtypes of PitNETs, DEGs were identified under the cutoff of adj.P.val F, G) Metascape enrichment analysis including pathways and GO term of 110 overlapping DEGs in Figure 1E. (H) Outline of bioinformatic GSEA analysis.

Figure 1. The protein-coding RNA landscape in 172 PitNETs. (A) Heatmap of unsupervised hierarchical clustering of the top 352 most variable genes among 31 somatotroph adenomas, 17 lactotroph adenomas, 79 gonadotroph adenomas and 45 corticotroph adenomas (|log2FC|>2, adj.P.val<0.001). Pathological and clinical annotations were provided. (BD) Volcano plots showing the significantly differentially expressed genes among somatotroph adenomas vs. 3 other subtypes of PitNETs (up: red, down: blue), all log2FC of DLK1 > 7. (E) Venn diagram showing the intersection of DEGs among somatotroph adenomas vs. 3 other subtypes of PitNETs, DEGs were identified under the cutoff of adj.P.val < 0.001. (F, G) Metascape enrichment analysis including pathways and GO term of 110 overlapping DEGs in Figure 1E. (H) Outline of bioinformatic GSEA analysis.