Research Paper Volume 13, Issue 1 pp 1440—1457

NLRC4 gene silencing-dependent blockade of NOD-like receptor pathway inhibits inflammation, reduces proliferation and increases apoptosis of dendritic cells in mice with septic shock

Silencing of NLRC4 inactivates the NLR pathway in mouse lung tissues. (A) The mRNA expression of NLRC4, NOD1, NOD2, RIP2, and NF-κB in DCs determined by RT-qPCR. (B) The protein expression of NLRC4, NOD1, NOD2, RIP2, and NF-κB normalized to GAPDH in DCs measured by Western blot analysis. (C) Western blot bands of NLRC4, NOD1, NOD2, RIP2, and NF-κB in different transfection groups. N = 10. * p p

Figure 3. Silencing of NLRC4 inactivates the NLR pathway in mouse lung tissues. (A) The mRNA expression of NLRC4, NOD1, NOD2, RIP2, and NF-κB in DCs determined by RT-qPCR. (B) The protein expression of NLRC4, NOD1, NOD2, RIP2, and NF-κB normalized to GAPDH in DCs measured by Western blot analysis. (C) Western blot bands of NLRC4, NOD1, NOD2, RIP2, and NF-κB in different transfection groups. N = 10. * p < 0.05 vs. the sham group. † p < 0.05 vs. the CLP and NC-siRNA groups. Data comparison among multiple groups was analyzed by one-way ANOVA, followed by Tukey’s post hoc test. The experiment was repeated 3 times independently.