Figure 3. Silencing of NLRC4 inactivates the NLR pathway in mouse lung tissues. (A) The mRNA expression of NLRC4, NOD1, NOD2, RIP2, and NF-κB in DCs determined by RT-qPCR. (B) The protein expression of NLRC4, NOD1, NOD2, RIP2, and NF-κB normalized to GAPDH in DCs measured by Western blot analysis. (C) Western blot bands of NLRC4, NOD1, NOD2, RIP2, and NF-κB in different transfection groups. N = 10. * p < 0.05 vs. the sham group. † p < 0.05 vs. the CLP and NC-siRNA groups. Data comparison among multiple groups was analyzed by one-way ANOVA, followed by Tukey’s post hoc test. The experiment was repeated 3 times independently.