Research Paper Volume 13, Issue 1 pp 1440—1457

NLRC4 gene silencing-dependent blockade of NOD-like receptor pathway inhibits inflammation, reduces proliferation and increases apoptosis of dendritic cells in mice with septic shock

NLRC4 downregulation alleviates inflammatory reaction and reduces inflammatory cell infiltration. (A) the levels of IL-1β, TNF-α and IL-6 in lung tissues of mice with septic shock detected by ELISA. (B) inflammatory cell infiltration evidenced by F4/80 and CD45 positive levels using TUNEL staining (scale bar = 25 μm). n = 10. * p p

Figure 4. NLRC4 downregulation alleviates inflammatory reaction and reduces inflammatory cell infiltration. (A) the levels of IL-1β, TNF-α and IL-6 in lung tissues of mice with septic shock detected by ELISA. (B) inflammatory cell infiltration evidenced by F4/80 and CD45 positive levels using TUNEL staining (scale bar = 25 μm). n = 10. * p < 0.05 vs. the sham group. † p < 0.05 vs. the CLP and NC-siRNA groups. Data comparison among multiple groups was analyzed by one-way ANOVA, followed by Tukey’s post hoc test. The experiment was repeated 3 times independently.