Figure 6. siRNA-mediated silencing of NLRC4 elevates DC cell apoptosis rate in mice with septic shock as evidenced by PI single staining and AnnexinV-FITC/PI double staining. (A, B), DC cycle distribution in the sham, CLP, NC-siRNA, and NLRC4-siRNA groups detected by PI single staining. (C, D) DC apoptosis in the sham, CLP, NC-siRNA, NLRC4-siRNA groups revealed by AnnexinV-FITC/PI double staining. The bone marrow-derived DCs from the same group of mice were adopted in the in vivo experiments. * p < 0.05 vs. the sham group. † p < 0.05 vs. the CLP and NC-siRNA groups. Data comparison among multiple groups was analyzed by one-way ANOVA, followed by Tukey’s post hoc test. The experiment was repeated 3 times independently.