Figure 5. Coiled-coil domain-containing protein 167 (CCDC167)-knockdown by shRNA significantly attenuated the proliferation of MCF-7 breast cancer cells. (A) The mRNA expression of CCDC167 was determined in a variety of breast cancer cell lines. (B) A qPCR analyzed CCDC167 mRNA expression in shCCDC167-knockdown cells. (C) Evaluation of the growth of shCCDC167-knockdown and vector control MCF-7 cells according to MTT assays. (D) A colony-formation assay determined proliferation rates of the MCF-7 vector control and stable shCCDC167-knockdown MCF-7 cells. (E) Statistical data of the colony-formation assay. Values are the average of assays performed in triplicate. The standard deviation is displayed using error bars (n=3). * p<0.05.