Research Paper Volume 13, Issue 3 pp 4242—4257

Oxaliplatin induces the PARP1-mediated parthanatos in oral squamous cell carcinoma by increasing production of ROS

Oxaliplatin caused the upregulation of nuclear PARP1, AIF and MIF, and nuclear translocation of AIF in OSCC cells. (A) The protein levels of PARP1, AIF, and MIF in the nucleus and cytoplasm of CAL27 cells treated with 0, 31.25, 62.5, and 125 μM oxaliplatin for 48 hours. (B) The protein level of PARP1, AIF, and MIF in the nucleus and cytoplasm of CAL27 cells treated with 125 μM oxaliplatin for 0, 12, 24, and 48 hours. (C) The protein levels of PARP1, AIF and MIF in the nucleus and cytoplasm of SCC25 cells treated with 0, 25, 50, and 100 μM oxaliplatin for 48 hours. (D) The protein levels of PARP1, AIF, and MIF in the nucleus and cytoplasm of SCC25 cells treated with 100 μM oxaliplatin for 0, 12, 24, and 48 hours. (E, F) Representative images of AIF immunofluorescence staining in CAL27 and SCC25 cells treated with oxaliplatin. Arrows indicates typical cells with AIF nuclear translocation. * p

Figure 4. Oxaliplatin caused the upregulation of nuclear PARP1, AIF and MIF, and nuclear translocation of AIF in OSCC cells. (A) The protein levels of PARP1, AIF, and MIF in the nucleus and cytoplasm of CAL27 cells treated with 0, 31.25, 62.5, and 125 μM oxaliplatin for 48 hours. (B) The protein level of PARP1, AIF, and MIF in the nucleus and cytoplasm of CAL27 cells treated with 125 μM oxaliplatin for 0, 12, 24, and 48 hours. (C) The protein levels of PARP1, AIF and MIF in the nucleus and cytoplasm of SCC25 cells treated with 0, 25, 50, and 100 μM oxaliplatin for 48 hours. (D) The protein levels of PARP1, AIF, and MIF in the nucleus and cytoplasm of SCC25 cells treated with 100 μM oxaliplatin for 0, 12, 24, and 48 hours. (E, F) Representative images of AIF immunofluorescence staining in CAL27 and SCC25 cells treated with oxaliplatin. Arrows indicates typical cells with AIF nuclear translocation. * p<0.05, **p<0.01.