Research Paper Volume 13, Issue 3 pp 4242—4257

Oxaliplatin induces the PARP1-mediated parthanatos in oral squamous cell carcinoma by increasing production of ROS

siRNA against PARP1 reversed oxaliplatin-induced parthanatos in OSCC cells. CAL27 and SCC25 cells were transfected with siRNA against PARP1 (siPARP1) or non-targeting siRNA (siNC), and they were further treated with or without oxaliplatin. (A, B) The cell death ratio based on LDH release assay of CAL27 and SCC25 cells. (C, D) The protein level of PARP1, AIF and MIF in the nucleus and cytoplasm of CAL27 cells. (E, F) Representative images of AIF immunofluorescence staining in CAL27 and SCC25 cells. Arrows indicates typical cells with AIF nuclear translocation. * p

Figure 6. siRNA against PARP1 reversed oxaliplatin-induced parthanatos in OSCC cells. CAL27 and SCC25 cells were transfected with siRNA against PARP1 (siPARP1) or non-targeting siRNA (siNC), and they were further treated with or without oxaliplatin. (A, B) The cell death ratio based on LDH release assay of CAL27 and SCC25 cells. (C, D) The protein level of PARP1, AIF and MIF in the nucleus and cytoplasm of CAL27 cells. (E, F) Representative images of AIF immunofluorescence staining in CAL27 and SCC25 cells. Arrows indicates typical cells with AIF nuclear translocation. * p<0.05, **p<0.01.