Research Paper Volume 13, Issue 3 pp 4242—4257

Oxaliplatin induces the PARP1-mediated parthanatos in oral squamous cell carcinoma by increasing production of ROS

NAC reversed oxaliplatin-induced parthanatos in OSCC cells. CAL27 and SCC25 cells were pretreated with or without antioxidant NAC for 1 hour, and then they were treated with oxaliplatin. (A, B) The cell death ratio based on LDH release assay of CAL27 and SCC25 cells. (C, D) The protein levels of PARP1, AIF and MIF in the nucleus and cytoplasm of CAL27 cells. (E, F) Representative images of AIF immunofluorescence staining in CAL27 and SCC25 cells. Arrows indicates typical cells with AIF nuclear translocation. * p

Figure 8. NAC reversed oxaliplatin-induced parthanatos in OSCC cells. CAL27 and SCC25 cells were pretreated with or without antioxidant NAC for 1 hour, and then they were treated with oxaliplatin. (A, B) The cell death ratio based on LDH release assay of CAL27 and SCC25 cells. (C, D) The protein levels of PARP1, AIF and MIF in the nucleus and cytoplasm of CAL27 cells. (E, F) Representative images of AIF immunofluorescence staining in CAL27 and SCC25 cells. Arrows indicates typical cells with AIF nuclear translocation. * p<0.05, **p<0.01.