Figure 1. Concentration screening of MPP+ and verification of CHIP plasmid and shRNA. (A) SH-SY5Y cells were treated with different concentrations of MPP+ to determine the optimum concentration. (B, C) Western blotting showed CHIP increased significantly after transfection with CHIP plasmid. (D, E) The expression of CHIP decreased under the action of shRNA. *P<0.05.