Research Paper Volume 13, Issue 3 pp 4299—4316

Overexpression of long noncoding RNA ANRIL inhibits phenotypic switching of vascular smooth muscle cells to prevent atherosclerotic plaque development in vivo

LncRNA-ANRIL regulates AMPK catalytic activity by binding to AMPK gamma subunit. (A) Cultured VSMCs were incubated with metformin (1 mM) for 12 hours. Cells were subjected to detect the binding of lncRNA-ANRIL or lncRNA-MALAT1 to AMPKγ subunit by using RNA-immunoprecipitation assay in (A) Quantitative analysis of the affinity between lncRNA-ANRIL and AMPKγ was performed. N is 5 in each group. *PB) Cultured VSMCs were infected with adenovirus expressing lncRNA-ANRIL for 48 hours. The binding of AMPKα or β to AMPKγ was assayed by IP.

Figure 4. LncRNA-ANRIL regulates AMPK catalytic activity by binding to AMPK gamma subunit. (A) Cultured VSMCs were incubated with metformin (1 mM) for 12 hours. Cells were subjected to detect the binding of lncRNA-ANRIL or lncRNA-MALAT1 to AMPKγ subunit by using RNA-immunoprecipitation assay in (A) Quantitative analysis of the affinity between lncRNA-ANRIL and AMPKγ was performed. N is 5 in each group. *P<0.05 vs. vehicle. (B) Cultured VSMCs were infected with adenovirus expressing lncRNA-ANRIL for 48 hours. The binding of AMPKα or β to AMPKγ was assayed by IP.