Figure 3. MiR-223 inhibited the migration of PDGF-stimulated HASMCs. Serum-deprived HASMCs were transfected with miR-223, miR-NC, and anti-miR-223 or anti-miR-NC for 24 h, followed by PDGF-BB stimulation for 24 h. (A) Transwell assay was conducted to assess cell migration. (B) The migratory ability of HASMCs was evaluated by wound healing assay. (C) Western blot assay was conducted to analyze the expressions of MMP-2 and MMP-9. β-actin was used as the endogenous control. The data are shown as mean ± SD of three separate experiments. *P < 0.05 compared with control group. #P < 0.05 compared with PDGF-BB group.