Research Paper Volume 13, Issue 6 pp 8055—8067

Silencing SIX1 by miR-7160 inhibits non-small cell lung cancer cell growth

miR-7160 overexpression provokes apoptosis in NSCLC cells. The primary NSCLC cells (pCan-1/pCan-2/pCan-3) or A549 cells, bearing the pre-miR-7160-expression lentiviral construct (“lv-pre-miR-7160”) or non-sense miRNA control lentiviral construct (“lv-miRC”), were cultured for 48h; Caspase activation (A, B, E), and cell apoptosis (C, D, F) were tested by the assays mentioned in the text, with results quantified. Data were presented as mean ± SD (n=5), and results normalized. *P

Figure 3. miR-7160 overexpression provokes apoptosis in NSCLC cells. The primary NSCLC cells (pCan-1/pCan-2/pCan-3) or A549 cells, bearing the pre-miR-7160-expression lentiviral construct (“lv-pre-miR-7160”) or non-sense miRNA control lentiviral construct (“lv-miRC”), were cultured for 48h; Caspase activation (A, B, E), and cell apoptosis (C, D, F) were tested by the assays mentioned in the text, with results quantified. Data were presented as mean ± SD (n=5), and results normalized. *P< 0.05 vs. “lv-miRC” cells. Experiments in this figure were repeated five times with similar results obtained.