Research Paper Volume 13, Issue 3 pp 4370—4387

Adipose-derived stromal cells improve functional recovery after spinal cord injury through TGF-β1/Smad3/PLOD2 pathway activation

ADSC co-culture promotes survival of mechanically injured neurons. (A) LDH release assay results from PC12 and primary rat neurons exposed to MI. (B) Real-time PCR analysis of PLOD2, MAP2, NSE, GAP43, and GFAP in MI-treated cells. (C) Western-blot analysis showing increased expression of PLOD2 and NSE in MI-treated cells co-cultured with ADSCs. Results presented as mean ± SD and evaluated with one-way ANOVA. *PP

Figure 2. ADSC co-culture promotes survival of mechanically injured neurons. (A) LDH release assay results from PC12 and primary rat neurons exposed to MI. (B) Real-time PCR analysis of PLOD2, MAP2, NSE, GAP43, and GFAP in MI-treated cells. (C) Western-blot analysis showing increased expression of PLOD2 and NSE in MI-treated cells co-cultured with ADSCs. Results presented as mean ± SD and evaluated with one-way ANOVA. *P<0.05, compared to control (no MI); #P<0.05, compared to MI. MI: mechanical injury.