Research Paper Volume 13, Issue 3 pp 4370—4387

Adipose-derived stromal cells improve functional recovery after spinal cord injury through TGF-β1/Smad3/PLOD2 pathway activation

PLOD2 inhibition attenuates ADSC-mediated neuronal protection following mechanical injury in vitro. (A) Light microscopy and MAP2 immunofluorescence imaging of PC12 cells 3 days after ADSC co-culture. Scale bars: 200 μm (upper row), 100 μm (middle and lower rows). (B) EdU assay results showing enhanced proliferation of rat cortical neurons after ADSC co-culture and reduced proliferation in the presence of the PLOD2 inhibitor minoxidil. Scale bar: 100 μm.

Figure 3. PLOD2 inhibition attenuates ADSC-mediated neuronal protection following mechanical injury in vitro. (A) Light microscopy and MAP2 immunofluorescence imaging of PC12 cells 3 days after ADSC co-culture. Scale bars: 200 μm (upper row), 100 μm (middle and lower rows). (B) EdU assay results showing enhanced proliferation of rat cortical neurons after ADSC co-culture and reduced proliferation in the presence of the PLOD2 inhibitor minoxidil. Scale bar: 100 μm.