Research Paper Volume 13, Issue 3 pp 4409—4427

Myeloid differentiation 2 deficiency attenuates AngII-induced arterial vascular oxidative stress, inflammation, and remodeling

MD2 is increased in aortas of Ang II induced mice. (A) MD2 protein levels in aortas of mice induced by Ang II were detected by western blotting. Panel (B) showed densitometric quantification (n = 10; **PC) Representative immunofluorescence staining of MD2 (red) and VSMCs marker α-SMA (green). Tissues were counterstained with DAPI (blue). Yellow arrows indicate co-location of MD2 and α-SMA stanning (scale bar = 50 μm). (D) Representative immunofluorescence staining of MD2 (red) and endothelial cells marker CD31 (green). Tissues were counterstained with DAPI (blue). Yellow arrows indicate co-location of MD2 and CD31 stanning (scale bar = 50 μm).

Figure 1. MD2 is increased in aortas of Ang II induced mice. (A) MD2 protein levels in aortas of mice induced by Ang II were detected by western blotting. Panel (B) showed densitometric quantification (n = 10; **P<0.01 compared to Vehicle). (C) Representative immunofluorescence staining of MD2 (red) and VSMCs marker α-SMA (green). Tissues were counterstained with DAPI (blue). Yellow arrows indicate co-location of MD2 and α-SMA stanning (scale bar = 50 μm). (D) Representative immunofluorescence staining of MD2 (red) and endothelial cells marker CD31 (green). Tissues were counterstained with DAPI (blue). Yellow arrows indicate co-location of MD2 and CD31 stanning (scale bar = 50 μm).