Research Paper Volume 13, Issue 3 pp 4468—4481

PRMT5 regulates colorectal cancer cell growth and EMT via EGFR/Akt/GSK3β signaling cascades

PRMT5 regulates Akt activation in human colorectal cancer cells. (A) Western blot analysis of phospho-Akt and total Akt protein expression level in HCT116 and SW480 cells. Representative data is shown. (B, C) The indicated proteins are quantified in HCT116 and SW480 cells. *P D) Western blot analysis of indicated protein expression level in HCT116 and SW480 cells. Representative data is shown. (E, F) The indicated proteins are quantified in HCT116 and SW480 cells. *P G) Western blot analysis of indicated protein expression level in HCT116 and SW480 cells. Representative data is shown. (H, I) The indicated proteins are quantified in HCT116 and SW480 cells. (J) Western blot analysis of indicated protein expression level in HCT116 cells. Representative data is shown. (K) The indicated proteins are quantified in HCT116 cells. *P

Figure 4. PRMT5 regulates Akt activation in human colorectal cancer cells. (A) Western blot analysis of phospho-Akt and total Akt protein expression level in HCT116 and SW480 cells. Representative data is shown. (B, C) The indicated proteins are quantified in HCT116 and SW480 cells. *P < 0.05 vs. Scr. (D) Western blot analysis of indicated protein expression level in HCT116 and SW480 cells. Representative data is shown. (E, F) The indicated proteins are quantified in HCT116 and SW480 cells. *P < 0.05 vs. Flag vector. (G) Western blot analysis of indicated protein expression level in HCT116 and SW480 cells. Representative data is shown. (H, I) The indicated proteins are quantified in HCT116 and SW480 cells. (J) Western blot analysis of indicated protein expression level in HCT116 cells. Representative data is shown. (K) The indicated proteins are quantified in HCT116 cells. *P < 0.05 vs. DMSO.