Research Paper Volume 13, Issue 3 pp 4482—4502

Identification of downstream signaling cascades of ACK1 and prognostic classifiers in non-small cell lung cancer

Knockdown (KD) of ACK1/TNK2 in A549 cells, followed by RNA-seq. ACK1 was silenced using three lentivirus-mediated shRNAs (A). The shRNA showing the highest efficiency of the ACK1 gene knockdown was used for subsequent experiments. Overlapping genes were identified in the negative control (NC) and KD groups (B). The volcano plot (C) indicated the significantly up- and downregulated genes after the silencing of ACK1 [absolute value of log2 (fold change) ≥1, PACK1/TNK2) were well clustered (D). Gene Ontology enrichment analysis of DEGs (E). KEGG pathway annotation of DEGs (F).

Figure 3. Knockdown (KD) of ACK1/TNK2 in A549 cells, followed by RNA-seq. ACK1 was silenced using three lentivirus-mediated shRNAs (A). The shRNA showing the highest efficiency of the ACK1 gene knockdown was used for subsequent experiments. Overlapping genes were identified in the negative control (NC) and KD groups (B). The volcano plot (C) indicated the significantly up- and downregulated genes after the silencing of ACK1 [absolute value of log2 (fold change) ≥1, P<0.001]. Based on differentially expressed genes (DEGs), three NC and three KD samples (shACK1/TNK2) were well clustered (D). Gene Ontology enrichment analysis of DEGs (E). KEGG pathway annotation of DEGs (F).