Figure 5. Analysis of the ACK1 signaling pathways. RNA-seq data of 188 lung cancer cell lines were retrieved from the CCLE database. GSEA was performed after dividing cell lines into ACK1high and ACK1low groups by the average ACK1 expression level (A). Principal component analysis (PCA) of the 57 hub genes (degree≥20) was carried out in the TCGA-LUAD (B) and TCGA-LUSC (C) versus normal tissues. The most significant 14-gene module was derived from DEGs, which captured 50 coexpressed genes in the TCGA-LUAD cohort (D). Most of the 64 genes were enriched in the ubiquitin-mediated proteolysis and the proteasome (E), which was validated by GSEA using CCLE lung cancer cell data (F).