Figure 5. In vitro inhibition of RhoA/ROCK in dKO macrophages reduced expression of SASP factors. (A) Cultured macrophages isolated from WT and dKO mice obtained by FACS with CD68 immunostaining to validate macrophage isolation in vitro. (B) qPCR results indicating dKO macrophages exhibit up-regulated expression of SASP factors that was reduced by treatment with the RhoA/ROCK inhibitor Y-27632 (10 μM, 2 days). * indicates p<0.05 vs. WT, * indicates p<0.05 vs. dKO.