Research Paper Volume 13, Issue 4 pp 5034—5054

Transcriptional dysregulation of TRIM29 promotes colorectal cancer carcinogenesis via pyruvate kinase-mediated glucose metabolism

TRIM29 promotes the malignant phenotype of CRC in vitro. (A) Western blotting was used to analyze the level of TRIM29 in CRC cell lines. (B) The efficiency of TRIM29 knockdown using siRNA in SW480 and DLD-1 cells and TRIM29 overexpression in DLD-1 and HCT116 cells. (C–E) SW480 and DLD-1 cells were transfected with siRNA, while HCT116 and DLD-1 cells were transfected with the TRIM29 overexpression plasmid. (C) After transfection for 24 h, an RTCA-MP proliferation assay was performed to measure cell proliferation. (D) Colony formation assay. (E) Transwell assays were performed to measure cell migration and invasion. The error bars represent the SEM. The statistical analysis was performed using two-tailed Student’s t-test. **P P P

Figure 3. TRIM29 promotes the malignant phenotype of CRC in vitro. (A) Western blotting was used to analyze the level of TRIM29 in CRC cell lines. (B) The efficiency of TRIM29 knockdown using siRNA in SW480 and DLD-1 cells and TRIM29 overexpression in DLD-1 and HCT116 cells. (CE) SW480 and DLD-1 cells were transfected with siRNA, while HCT116 and DLD-1 cells were transfected with the TRIM29 overexpression plasmid. (C) After transfection for 24 h, an RTCA-MP proliferation assay was performed to measure cell proliferation. (D) Colony formation assay. (E) Transwell assays were performed to measure cell migration and invasion. The error bars represent the SEM. The statistical analysis was performed using two-tailed Student’s t-test. **P < 0.01, ***P < 0.001, ****P < 0.0001. Every experiment repeated at least 3 times.