Figure 6. TRIM29 regulates glycolytic metabolism and the aggressive phenotype in CRC by regulating PKM1. (A, B) After transfection with NC or Si2 in SW480 and HT29 cell lines, (A) The extracellular acidification rate (ECAR) and (B) the oxygen consumption rate (OCR) of cells was measured. (C–F) Cells transfected with GV230 vector plus Ez-M98 vector, TRIM29 plasmid plus Ez-M98 vector, TRIM29 plasmid plus PKM1 plasmid. (C) The ECAR and (D) OCR of cells were measured. (E) Growth was determined using the RTCA-MP system. (F) Migration and invasion were measured using a transwell assay. The statistical analysis was performed using the two-tailed Student’s t-test. **P < 0.01, ***P < 0.001. Every experiment repeated at least 3 times.