Research Paper Volume 13, Issue 3 pp 4522—4551

Hsa_circ_0000199 facilitates chemo-tolerance of triple-negative breast cancer by interfering with miR-206/613-led PI3K/Akt/mTOR signaling

Sponged relationship between hsa

Figure 3. Sponged relationship between hsa_circ_0000199 and miR-613/miR-206 in triple-negative breast cancer (TNBC). (A) Hsa_circ_0000199 expression in MDA-MB-231 and MDA-MB-468 cell lines was determined after transfection of si-hsa_circ_0000199. ***: P<0.001. (B) MiR-613 and miR-206 expressions in MDA-MB-231 and MDA-MB-468 cell lines were compared between si-NC group and si-hsa_circ_0000199 group. ***: P<0.001. (C) MiR-613 and miR-206 expressions were assessed after transfection of their respective inhibitors into MDA-MB-231 and MDA-MB-468 cell lines. ***: P<0.001. (D) Hsa_circ_0000199 expression in MDA-MB-231 and MDA-MB-468 cell lines was evaluated after transfection of miR-613/miR-206 inhibitor. (E) Hsa_circ_0000199 sponged miR-613 in certain sites, and luciferase activity of MDA-MB-231 and MDA-MB-468 cell lines was compared between pGL3-hsa_circ_0000199 Wt+miR-613 mimic group and pGL3-hsa_circ_0000199 Wt+miR-NC group. ***: P<0.001. (F) MiR-206 was sponged by hsa_circ_0000199, and luciferase activity of MDA-MB-231 and MDA-MB-468 cell lines was determined after co-transfection of pGL3-hsa_circ_0000199 Wt and miR-206 mimic/miR-NC. ***: P<0.001.