Figure 6. Validation of hub genes by cell experiments in vitro. (A) Identification of differentially expressed genes by qPCR assay in both normal endometrial epithelial cells and endometrial cancer cell lines. (B) Western blot assay showing the expression of TICRR and PPIF in EC cell lines and normal endometrial epithelial cells. (C) The effect of different siRNA on TICRR and PPIF gene silence by western blotting respectively. (D) TICRR and PPIF expression were examined by RT-PCR after transfected with siRNA for 24h. (E) Relevant molecular targets were verified by qRCR after transfection of siTICRR and siPPIF for 24hrs respectively. (F) Cell growth and clone formation of siTICRR transfected cells with or without MPA. (G) Cell growth and clone formation of siPPIF transfected cells with or without MPA. Data were shown as mean ± SD; *p < 0.05; **p < 0.01; ***p < 0.001.