Research Paper Volume 13, Issue 3 pp 4564—4589

Overexpression of TICRR and PPIF confer poor prognosis in endometrial cancer identified by gene co-expression network analysis

IHC staining and functional verification of TICRR and PPIF genes. (A), The expression of TICRR and PPIF in endometrial cancer and paracancer samples were assessed using IHC staining and the results of statistical analysis. (B) Analysis of apoptosis by Flow cytometry after transfection with siTICRR and siPPIF respectively. (C) Hec-1B-siTICRR cells were subjected to migration and invasion assays in the presence or absence of MPA. (D) Migration and invasion ability of siPPIF transfected cells in the presence or absence of MPA. (E) Wound-healing assays for Hec-1B-siTICRR cells. (F) Wound-healing assays for Hec-1B-siPPIF cells. Data were shown as mean ± SD; *p

Figure 7. IHC staining and functional verification of TICRR and PPIF genes. (A), The expression of TICRR and PPIF in endometrial cancer and paracancer samples were assessed using IHC staining and the results of statistical analysis. (B) Analysis of apoptosis by Flow cytometry after transfection with siTICRR and siPPIF respectively. (C) Hec-1B-siTICRR cells were subjected to migration and invasion assays in the presence or absence of MPA. (D) Migration and invasion ability of siPPIF transfected cells in the presence or absence of MPA. (E) Wound-healing assays for Hec-1B-siTICRR cells. (F) Wound-healing assays for Hec-1B-siPPIF cells. Data were shown as mean ± SD; *p < 0.05; **p < 0.01; ***p < 0.001.