Research Paper Volume 13, Issue 3 pp 4617—4633

LNK promotes granulosa cell apoptosis in PCOS via negatively regulating insulin-stimulated AKT-FOXO3 pathway

The incidence of insulin resistance in PCOS patients and control group, and the expression of LNK in granulosa cells from PCOS and control group. (A) PCOS patients had a higher incidence of insulin resistance. GLUT4 mRNA level was increased in PCOS group. Results are mean±SD. *p B) RT-PCR and western blot results showed higher levels of LNK mRNA and protein expression in granulosa cells in PCOS group compared with control group. Results are mean±SEM. *p p C) Pearson correlation analysis showed a positive correlation between LNK (relative mRNA level, fold of GAPDH) and clinical insulin resistance parameters in the total population.

Figure 1. The incidence of insulin resistance in PCOS patients and control group, and the expression of LNK in granulosa cells from PCOS and control group. (A) PCOS patients had a higher incidence of insulin resistance. GLUT4 mRNA level was increased in PCOS group. Results are mean±SD. *p < 0.05 vs con. (B) RT-PCR and western blot results showed higher levels of LNK mRNA and protein expression in granulosa cells in PCOS group compared with control group. Results are mean±SEM. *p < 0.05, **p < 0.01 vs. data of control group. (C) Pearson correlation analysis showed a positive correlation between LNK (relative mRNA level, fold of GAPDH) and clinical insulin resistance parameters in the total population.