Research Paper Volume 12, Issue 24 pp 24894—24913

Obesity induced by high-fat diet is associated with critical changes in biological and molecular functions of mesenchymal stromal cells present in visceral adipose tissue

Figure 1. Experimental design. Mice were fed with a normal diet (ND) and a high-fat diet (HFD) for 20 weeks. One week before animal euthanasia, GTT and ITT tests were conducted. The MSCs isolated from visceral adipose tissue were characterized by flow cytometry, senescence-associated β-galactosidase assay, annexin V staining, and cell analysis performed at passage 3. At passage 3, for each experimental group, we collected total cellular protein content and secretomes as well as performed LC-MS/MS analysis.