Figure 2. High fat diet outcomes. (A) Average body weight of animals through 20 weeks. The histogram shows the weight of the five mice fed with HFD and the five mice with ND for 20 weeks. Data are shown with standard deviation (SD) n=5 *p<0.05, **p<0.01. (B) Insulin tolerance tests (ITT) and glucose tolerance tests (GTT). The graphs show blood glucose levels (mg/dl) in HFD- and ND-treated mice. (C) Flow cytometry results on visceral adipose-derived MSCs. The picture shows a representative example of flow cytometry analysis carried out on P0 MSCs obtained from an ND-treated mouse. Cells were positive for MSC markers CD29 and CD90 and negative for MSC marker CD45. The same results were obtained with cells isolated from HFD-treated mice (data not shown).