Biological assays in HFD- and ND-MSCs. (A) The histogram shows the mean percentage value of senescent cells, determined by beta-galactosidase assay. Data are expressed with SD (n = 5) ****p<0.001. Representative images of senescent cells that are stained blue are shown. (B) The histogram shows the mean percentage of annexin V-positive cells. Data are expressed with standard deviation (n = 5) **p<0.01. (C) Cell proliferation was evaluated by MTT colorimetric assay. The graph shows data coming from HFD and ND samples. (D) Representative cell cycle analysis of MSCs harvested from HFD- and ND-treated mice. Data are expressed with SD (n = 5) *p<0.05, **p<0.01, ***p<0.001.