Research Paper Volume 13, Issue 4 pp 5055—5068

MicroRNA-29a-3p delivery via exosomes derived from engineered human mesenchymal stem cells exerts tumour suppressive effects by inhibiting migration and vasculogenic mimicry in glioma

Glioma formed VM structures and showed aberrantly low expression level of miR-29a-3p. (A) Quantification of miR-29a-3p expression levels in gliomas of different subtypes in the TCGA database. Data are shown as the mean±SD, n=204, one-way ANOVA (*, P B) miR-29a-3p expression in NHA, U87 and A172 cells. Data are shown as the mean±SD, n=3, one-way ANOVA (*, P C) Representative images of CD34-PAS IHC staining of VM structures (red arrows; scale bar, 100 μm) in normal brain specimens (n=2) and in gliomas of different grades (n=45). Epithelium-lined vessels (CD34+/PAS+) were set as positive control (green arrows; scale bar, 100 μm).

Figure 1. Glioma formed VM structures and showed aberrantly low expression level of miR-29a-3p. (A) Quantification of miR-29a-3p expression levels in gliomas of different subtypes in the TCGA database. Data are shown as the mean±SD, n=204, one-way ANOVA (*, P < 0.05). (B) miR-29a-3p expression in NHA, U87 and A172 cells. Data are shown as the mean±SD, n=3, one-way ANOVA (*, P < 0.05). (C) Representative images of CD34-PAS IHC staining of VM structures (red arrows; scale bar, 100 μm) in normal brain specimens (n=2) and in gliomas of different grades (n=45). Epithelium-lined vessels (CD34+/PAS+) were set as positive control (green arrows; scale bar, 100 μm).