Research Paper Volume 13, Issue 4 pp 5055—5068

MicroRNA-29a-3p delivery via exosomes derived from engineered human mesenchymal stem cells exerts tumour suppressive effects by inhibiting migration and vasculogenic mimicry in glioma

miR-29a-3p inhibited migration and VM formation in glioma cells. (A) The effect of miR-29a-3p on cell movement was assessed using transwell migration assays (scale bar, 100 μm; n=3). (B) Quantification of transwell migration assays in (A). Data are shown as the mean±SD, n=3, one-way ANOVA (*, P C) Effect of miR-29a-3p on VM formation ability (scale bar, 200 μm; n=3). (D) Quantification of relative VM number in (C). Data are shown as the mean±SD, n=3, one-way ANOVA (*, P E) Western blot analysis of protein levels of N-cadherin, MMP9 and LAMB2 in A172 and U87 cells. GAPDH was used as a whole-cell protein loading control. Results are from three independent experiments.

Figure 2. miR-29a-3p inhibited migration and VM formation in glioma cells. (A) The effect of miR-29a-3p on cell movement was assessed using transwell migration assays (scale bar, 100 μm; n=3). (B) Quantification of transwell migration assays in (A). Data are shown as the mean±SD, n=3, one-way ANOVA (*, P < 0.05). (C) Effect of miR-29a-3p on VM formation ability (scale bar, 200 μm; n=3). (D) Quantification of relative VM number in (C). Data are shown as the mean±SD, n=3, one-way ANOVA (*, P < 0.05). (E) Western blot analysis of protein levels of N-cadherin, MMP9 and LAMB2 in A172 and U87 cells. GAPDH was used as a whole-cell protein loading control. Results are from three independent experiments.