Research Paper Volume 13, Issue 5 pp 6606—6624

Coexpression of HOXA6 and PBX2 promotes metastasis in gastric cancer

HOXA6 and PBX2 promote the migration and invasion of gastric cancer cells. (A) The expression of vector (group 1), or HOXA6 (group 2), or PBX2 (group 3), or HOXA6 plus PBX2 (group 4) in AGS stable transfectants cells detecting by western blot assay. (B) The expression of vector plus scrambled siRNA (Scr siRNA, group 1), or HOXA6 plus Scr siRNA (group 2), or vector plus PBX2 siRNA 2 (group 3), or HOXA6 plus PBX2 siRNA 2 (Group 4) in AGS transfectants cells at protein level as detected. (C) The expression of vector plus Scr siRNA (group 1), or PBX2 plus Scr siRNA (group 2), or vector plus HOXA6 siRNA 2 (group 3), or PBX2 plus HOXA6 siRNA 2 (Group 4) in AGS transfectants cells measuring by western blot analysis. (D) The expression of Scr siRNA (group 1), or Src siRNA plus PBX2 siRNA 2 (group 2), or HOXA6 siRNA 2 plus Src siRNA (group 3), or HOXA6 siRNA 2 + PBX2 siRNA 2 was subjected to western blot analysis. (E–L) GC cells were evaluated by wound healing and transwell invasion assays. Photographs show cell migration into the wounded area, and the data shows the percentage of wound closure in the histogram. Photographs show the cells that travelled through the micropore membrane. **, p M) The F-actin filaments of GC cells staining with rhodamine-phallotoxin for 48h were observed by fluorescence microscopy. Scale bars in M represent 20 μm.

Figure 7. HOXA6 and PBX2 promote the migration and invasion of gastric cancer cells. (A) The expression of vector (group 1), or HOXA6 (group 2), or PBX2 (group 3), or HOXA6 plus PBX2 (group 4) in AGS stable transfectants cells detecting by western blot assay. (B) The expression of vector plus scrambled siRNA (Scr siRNA, group 1), or HOXA6 plus Scr siRNA (group 2), or vector plus PBX2 siRNA 2 (group 3), or HOXA6 plus PBX2 siRNA 2 (Group 4) in AGS transfectants cells at protein level as detected. (C) The expression of vector plus Scr siRNA (group 1), or PBX2 plus Scr siRNA (group 2), or vector plus HOXA6 siRNA 2 (group 3), or PBX2 plus HOXA6 siRNA 2 (Group 4) in AGS transfectants cells measuring by western blot analysis. (D) The expression of Scr siRNA (group 1), or Src siRNA plus PBX2 siRNA 2 (group 2), or HOXA6 siRNA 2 plus Src siRNA (group 3), or HOXA6 siRNA 2 + PBX2 siRNA 2 was subjected to western blot analysis. (EL) GC cells were evaluated by wound healing and transwell invasion assays. Photographs show cell migration into the wounded area, and the data shows the percentage of wound closure in the histogram. Photographs show the cells that travelled through the micropore membrane. **, p < 0.05; ***, p < 0.01; ****, p < 0.001. (M) The F-actin filaments of GC cells staining with rhodamine-phallotoxin for 48h were observed by fluorescence microscopy. Scale bars in M represent 20 μm.