Figure 2. Effect of liraglutide on H/R-induced cell death. H9C2 cells were pretreated with 200 nmol/L liraglutide or vehicle 30 min before H/R treatment, and stained with Hoechst 33342 (A) 24 h later. The number of Hoechst-positive (B) was calculated. Percentage of death cells were counted by Automated Cell Counter (C). The number of cells in Q1 (AV-/PI+, The necrotic cells), Q2 (AV+/PI+, the late phase apoptotic cells), Q3 (AV-/PI-, normal cell) and Q4 (AV+/PI-, the early phase apoptotic cells) were also analyzed using Flow cytometry (D, E).