Research Paper Volume 13, Issue 5 pp 6625—6633

Liraglutide preconditioning attenuates myocardial ischemia/ reperfusion injury via homer1 activation

Effect of Homer1 overexpression on liraglutide-induced cardiomyocyte protection. H9C2 cell were transfected with LV-H1 or LV-Con for 72 h before H/R treatment, and the expression of Homer1 was examined by immunofluorescence staining (A, B). The cell viability (C) and LDH release (D) were assayed 24 h later, and the Ca2+ concentration in ER ([Ca2+]ER) was measured at 5, 10 and 15 min after LV-Con and LV-H1 (E).

Figure 5. Effect of Homer1 overexpression on liraglutide-induced cardiomyocyte protection. H9C2 cell were transfected with LV-H1 or LV-Con for 72 h before H/R treatment, and the expression of Homer1 was examined by immunofluorescence staining (A, B). The cell viability (C) and LDH release (D) were assayed 24 h later, and the Ca2+ concentration in ER ([Ca2+]ER) was measured at 5, 10 and 15 min after LV-Con and LV-H1 (E).