Research Paper Volume 13, Issue 5 pp 6625—6633

Liraglutide preconditioning attenuates myocardial ischemia/ reperfusion injury via homer1 activation

Effect of Homer1 knock out on liraglutide-induced cardiomyocyte protection. H9C2 cell were transfected with si-Homer1, si-NC or vehicle for 72 h before H/R treatment, and the expression of Homer1 was examined by immunofluorescence staining (A, B). The cell viability (C) and LDH release (D) were assayed 24 h later, and the Ca2+ concentration in ER ([Ca2+]ER) was measured at 5, 10 and 15 min after si-Homer1 and si-NC (E).

Figure 6. Effect of Homer1 knock out on liraglutide-induced cardiomyocyte protection. H9C2 cell were transfected with si-Homer1, si-NC or vehicle for 72 h before H/R treatment, and the expression of Homer1 was examined by immunofluorescence staining (A, B). The cell viability (C) and LDH release (D) were assayed 24 h later, and the Ca2+ concentration in ER ([Ca2+]ER) was measured at 5, 10 and 15 min after si-Homer1 and si-NC (E).