Research Paper Volume 12, Issue 24 pp 26236—26247

Exosomal linc-FAM138B from cancer cells alleviates hepatocellular carcinoma progression via regulating miR-765

Figure 3. Exosomal linc-FAM138B inhibited the proliferation, migration and invasion of HCC cells. Cancer cells were isolated from HCC tissues. (A) Cancer cells were transfected with linc-FAM138B or NC, qRT-PCR was to determine transfection efficiency. (B) Exosomes were isolated from cancer cells after transfection, qRT-PCR was to determine linc-FAM138B expression in isolated exosomes. (C) SK-HEP-1 and HepG2 cells were incubated with isolated exosomes, and the expression of linc-FAM138B in SK-HEP-1 and HepG2 cells was detected using qRT-PCR. (D) MTT assay was to detected proliferation of SK-HEP-1 and HepG2 cells. (E, F) Wound healing assay was to evaluate migration of SK-HEP-1 and HepG2 cells. Scale bar, 60 μm. (G, H). Transwell assay was to examine invasion of SK-HEP-1 and HepG2 cells. Scale bar, 60 μm. Data are mean ± SD; *P < 0.05. Data among multiple groups were analyzed by one-way ANOVA, followed by a Tukey post hoc test. The experiment was repeated in triplicate.