Research Paper Volume 12, Issue 24 pp 26236—26247

Exosomal linc-FAM138B from cancer cells alleviates hepatocellular carcinoma progression via regulating miR-765

Figure 6. Exo-FAM138B suppressed HCC growth in vivo. PKH67 labeled linc-FAM138 was transfected into tumor cells, then exosomes from tumor cells were isolated. SK-HEP-1 and HepG2 cells were subcutaneously injected into nude mice. And a dosage of 5 mg exosomes or 10 μl saline was administered into mice via tail vein injection once every 3 days for 2 weeks. (A) Immunofluorescence assay showed a prominent fluorescence intensity of PKH67 in isolated tumor tissues. Scale bar, 100 μm. (B, C) Tumor volume was measured every 7 days. (D, E) Tumors was isolated after 28 days of SK-HEP-1 and HepG2 cells injection, and photos for representative tumors. The ratio of tumor wight to body weight of mice was calculated. (F) The level of linc-FAM138B in isolated tumors were detected by qRT-PCR. (G) The mRNA level of miR-765 in isolated tumors was tested. Data are mean ± SD; *P < 0.05. Data among multiple groups were analyzed by one-way ANOVA, followed by a Tukey post hoc test. The experiment was repeated in triplicate.