Figure 1. Notch1 signaling was activated in murine AAA. (A) Quantitative real-time PCR of Notch1, DLL4, hes1 and hey1 mRNA expression in aortas of mice with control (saline), AngII, AngII plus IMD and AngII plus DAPT. (B) Western blot analysis of hes1 and Notch1 signaling pathway marker NICD in aortas with Control, AngII, AngII+IMD and AngII+DAPT treatment. β-actin was a control for protein loading. Results are from 1 representative experiment of 3. (C) Quantification is shown as a ratio of β-actin expression. (D, E) Immunohistochemistry of the protein expression of NICD and hes1 in aortas of mice. Scale bar, 500 μm, 100 μm. Boxes and arrows show enlarged areas. Data are mean ± SD. *P<0.05, **P<0.01 vs. Control. #P<0.05, ##P<0.01 vs. AngII.