Figure 4. IMD inhibited macrophage-mediated inflammation via Notch1 signaling. (A) Quantitative real-time PCR of mRNA levels of IL-6, MCP-1, IFN-γ and TNF-α in aortas of Control, AngII, AngII+IMD and AngII+DAPT mice. (B) Representative immunofluorescence analysis of the protein expression of CD68 (green) and DAPI (blue) in aortas of mice. Scale bar, 100 μm. (C) Scratch-wound test to detect the transfer ability of mouse peritoneal macrophages in vitro. PBS, control group; LPS, stimulated group (100 ng/mL, 12 h). IMD was pretreated with a concentration gradient (1, 10, 100 nmol/L, 1 h). Cell migration distance was observed at 12 h after scratch wounding. Scale bar, 200 μm. n=3. (D) Quantification of (B). n=3, Data are mean ± SD. **P<0.01 vs. Control. ##P<0.01 vs. AngII. (E) Quantification of scratch-wound test. n=3, Data are mean ± SD. *P<0.05 vs. Control. #P<0.05 vs. LPS.