Figure 6. IMD inhibited macrophage M1 polarization. Control, saline treatment group; LPS+ IFN-γ, M1-type macrophage induction group; IFN-γ+IMD, M1-type macrophages pretreated with IMD; IL-4, M2-type macrophage induction group; IL-4+IMD, M2-type macrophages pretreated with IMD. Quantitative real-time PCR analysis of mRNA levels of (A) M1-type macrophage marker IL-12, IL-1β, TNF-α and iNOS in BMDMs and (B) M2-type macrophage marker Arg1, IL-10 and CD206 in BMDMs. Western blot analysis of (C, D) M1-type macrophage markers IL-12, IL-1β and CD16 and (E, F) M2-type macrophage markers Arg1, IL-10 and CD206 in BMDMs. β-actin was a control for protein loading. n=3, Data are mean ± SD. *P<0.05 vs. Control. #P<0.05 vs. IFN-γ.