Research Paper Volume 13, Issue 8 pp 11010—11025

Obacunone protects retinal pigment epithelium cells from ultra-violet radiation-induced oxidative injury

Obacunone attenuates UVR-induced cytotoxicity in RPE cells. ARPE-19 cells (A, B, E) or the primary murine RPE cells (C, D, F) were pretreated for 60 min with applied concentration of obacunone, cells were then subjected to UV radiation (UVR, UVA2+B, 30 mJ/cm2) or H2O2 (400 μM), and were further cultured for 48h; Cell viability was tested by CCK-8 assay (A, C), with cell death examined by LDH release in the conditional medium (B, D, E, F); Data were presented as mean ± SD (n=5). “Mock” stands for no UVR stimulation. “Veh” stands for the vehicle control. “C” stands for untreated control. *p vs. “Mock”/“C” treatment. #p vs. UVR/ H2O2 only treatment. Experiments were repeated three times, with similar results obtained.

Figure 1. Obacunone attenuates UVR-induced cytotoxicity in RPE cells. ARPE-19 cells (A, B, E) or the primary murine RPE cells (C, D, F) were pretreated for 60 min with applied concentration of obacunone, cells were then subjected to UV radiation (UVR, UVA2+B, 30 mJ/cm2) or H2O2 (400 μM), and were further cultured for 48h; Cell viability was tested by CCK-8 assay (A, C), with cell death examined by LDH release in the conditional medium (B, D, E, F); Data were presented as mean ± SD (n=5). “Mock” stands for no UVR stimulation. “Veh” stands for the vehicle control. “C” stands for untreated control. *p < 0.05 vs. “Mock”/“C” treatment. #p < 0.05 vs. UVR/ H2O2 only treatment. Experiments were repeated three times, with similar results obtained.