Research Paper Volume 13, Issue 8 pp 11010—11025

Obacunone protects retinal pigment epithelium cells from ultra-violet radiation-induced oxidative injury

Obacunone attenuates UVR-induced apoptosis in RPE cells. APRE-19 cells (A–D) or primary murine RPE cells (E) were pretreated for 60 min with obacunone (25 μM), cells were then subjected to UV radiation (UVR, UVA2+B, 30 mJ/cm2), and further cultured for 36h, relative caspase-3/-9 activity (A, B) and apoptosis activation (C–E) were tested by the listed assays. Data were presented as mean ± SD (n=5). “Mock” stands for no UVR stimulation. “Veh” stands for the vehicle control. *p vs. “Mock” treatment. #p vs. “UVR” only treatment (with vehicle pretreatment). Experiments were repeated three times, with similar results obtained. Bar= 100 μm (D).

Figure 2. Obacunone attenuates UVR-induced apoptosis in RPE cells. APRE-19 cells (AD) or primary murine RPE cells (E) were pretreated for 60 min with obacunone (25 μM), cells were then subjected to UV radiation (UVR, UVA2+B, 30 mJ/cm2), and further cultured for 36h, relative caspase-3/-9 activity (A, B) and apoptosis activation (CE) were tested by the listed assays. Data were presented as mean ± SD (n=5). “Mock” stands for no UVR stimulation. “Veh” stands for the vehicle control. *p < 0.05 vs. “Mock” treatment. #p < 0.05 vs. “UVR” only treatment (with vehicle pretreatment). Experiments were repeated three times, with similar results obtained. Bar= 100 μm (D).