Research Paper Volume 13, Issue 8 pp 11010—11025

Obacunone protects retinal pigment epithelium cells from ultra-violet radiation-induced oxidative injury


Figure 3. Obacunone ameliorates UVR-induced oxidative injury in RPE cells. ARPE-19 cells (AD) or the primary murine RPE cells (E and F) were pretreated for 60 min with obacunone (25 μM), cells were then subjected to UV radiation (UVR, UVA2+B, 30 mJ/cm2), and were further cultured for 12h, ROS production (CellROX intensity, A, E), mitochondrial depolarization (JC-1 green monomers fluorescence intensity, B and F), lipid peroxidation (measuring relative TBAR activity, C) and DNA damage (measuring ssDNA contents, D) were tested, and results were quantified. Data were presented as mean ± SD (n=5). “Mock” stands for no UVR stimulation. “Veh” stands for the vehicle control. *p < 0.05 vs. “Mock” treatment. # p < 0.05 vs. “UVR” only treatment (with vehicle pretreatment). Experiments were repeated three times, with similar results obtained.