Figure 2. MIR205HG directly binds to miR-299-3p. (A) The predicted target binding site for miR-299-3p in the 3'UTR of lncRNA MIR205HG based on the miRDB and starBase database analysis. (B) Dual luciferase reporter assay results show the luciferase activity in A375 cells co-transfected with plasmid containing wild-type (WT) or mutant (MT) MIR205HG 3′-UTR and miR-299-3p. (C) QRT-PCR analysis shows miR-299-3p in A375 cells transfected with miR-299-3p mimics or inhibitor for 24 h and the corresponding control A375 cells. (D) RNA pulldown assay results show the miR-299-3p levels associated with the biotinylated MIR205HG and control probes. Note: All experiments were performed thrice. **P<0.01 vs. control.