Research Paper Volume 13, Issue 4 pp 5297—5311

LncRNA MIR205HG regulates melanomagenesis via the miR-299-3p/VEGFA axis

In vivo growth of melanoma is inhibited by MIR205HG silencing. (A) Tumor volumes in nude mice subcutaneously injected with control and MIR205HG shRNA2-transfected A375 cells. Tumor volumes were measured weekly. (B) Representative images show the xenograft tumors in nude mice at 4 weeks after subcutaneously injecting control or MIR205HG shRNA2-transfected A375 cells. (C) Tumor weights in nude mice subcutaneously injected with control and MIR205HG shRNA2-transfected A375 cells (n=4 per group). (D, E) Western blot analysis shows the expression levels of VEGFA and cleaved caspase3 in xenograft tumor tissues harvested from nude mice subcutaneously injected with control and MIR205HG shRNA2-transfected A375 cells. The relative protein expression levels were quantified by normalizing to endogenous β-actin as the loading control. All experiments were performed thrice. (F) The expressions of active caspase 3 and VEGFA in tumor tissues of mice were detected by IHC staining. Black arrows indicate the positive cells after IHC staining. **P

Figure 6. In vivo growth of melanoma is inhibited by MIR205HG silencing. (A) Tumor volumes in nude mice subcutaneously injected with control and MIR205HG shRNA2-transfected A375 cells. Tumor volumes were measured weekly. (B) Representative images show the xenograft tumors in nude mice at 4 weeks after subcutaneously injecting control or MIR205HG shRNA2-transfected A375 cells. (C) Tumor weights in nude mice subcutaneously injected with control and MIR205HG shRNA2-transfected A375 cells (n=4 per group). (D, E) Western blot analysis shows the expression levels of VEGFA and cleaved caspase3 in xenograft tumor tissues harvested from nude mice subcutaneously injected with control and MIR205HG shRNA2-transfected A375 cells. The relative protein expression levels were quantified by normalizing to endogenous β-actin as the loading control. All experiments were performed thrice. (F) The expressions of active caspase 3 and VEGFA in tumor tissues of mice were detected by IHC staining. Black arrows indicate the positive cells after IHC staining. **P<0.01 vs. control.