Priority Research Paper Volume 12, Issue 24 pp 24504—24521

Neuroprotective effects and mechanisms of action of nicotinamide mononucleotide (NMN) in a photoreceptor degenerative model of retinal detachment


Figure 3.

Increased NAD+ and SIRT1 levels after retinal detachment (RD) with NMN supplementation. Significant increase of NAD+ levels in the NMN-treated detached retinas compared to the vehicle and the attached retinas at 24h (A) and three days (B) after RD. NAD+ levels in vehicle-treated detached retinas were not lower compared to untreated controls. N = 8 to 11 eyes per group. (C) Upregulation of SIRT1 protein levels in NMN-treated retinas seen at 24h and 7 days post RD. SIRT1 levels were comparable among groups at three days post RD. N = 3 to 7 eyes per group. Statistical significance was analyzed with one-way ANOVA followed by Tukey-Kramer adjustments. *p<0.05. **p<0.01. ***p<0.001. Data are mean ± SEM.