Figure 6. Protective effects of NMN and SIRT1 activation to 661W cells in RAW264.7 conditioned media (CM). (A) NMN administration significantly increased the cell viability of 661W cells in both the LPS treated and untreated RAW264.7 CM. N = 3 to 4 per group. (B) SRT1720 also showed a protective effect to 661W cells in the LPS treated RAW264.7 CM. N = 4 per group. The CM from neither NMN (C) or SRT1720 (D) treated RAW264.7 cells had protective effects to 661W cells. N = 4 to 6 per group. LPS can induce significant increases in both TNF-α (E) and IL-1β (F) expressions, which were not altered by NMN administration. N = 3 per group. (G) SIRT1 siRNA in 661W cells significantly reduced cell viability in the LPS stimulated RAW264.7 CM. #p<0.05 to LPS CM treated group in SC siRNA condition. N = 3 to 4 per group. Statistical significance was analyzed with the unpaired Student's t-test between each treatment and its control, or with one-way ANOVA followed by Tukey-Kramer adjustments. *p<0.05. **p<0.01. ***p<0.001. Data are mean ± SEM.