Research Paper Volume 13, Issue 1 pp 89—103

Further assessment of Salvia haenkei as an innovative strategy to counteract skin photo-aging and restore the barrier integrity

Effect of Salvia haenkei extract 0.01-0.1-1-10 μg/ml on ROS generation in HaCaT cells in basal and oxidative stress conditions. ROS were measured after 3-24 hours of incubation with SH under basal condition (A) or after H2O2 exposure (B, C). Figure (C) represents the effect of H2O2 stimulation of untreated cells, while figure (B) represents the effect of H2O2 stimulation of treated cells. NAC 5 mM is used as positive control. Data are expressed as mean ± SEM of fluorescence intensity (FI) of treated cells related to control. n = 3-4 experiments. *pppvs control.

Figure 2. Effect of Salvia haenkei extract 0.01-0.1-1-10 μg/ml on ROS generation in HaCaT cells in basal and oxidative stress conditions. ROS were measured after 3-24 hours of incubation with SH under basal condition (A) or after H2O2 exposure (B, C). Figure (C) represents the effect of H2O2 stimulation of untreated cells, while figure (B) represents the effect of H2O2 stimulation of treated cells. NAC 5 mM is used as positive control. Data are expressed as mean ± SEM of fluorescence intensity (FI) of treated cells related to control. n = 3-4 experiments. *p<0.05, **p<0.01, ***p<0.001 treatment vs control.